![]() “Today’s action is a strategic redirection of our path forward – largely back to the core of who we were when we set out on this journey.” “The Urgent Company succeeded in doing exactly what it was created to do – ignite a new category, helping our B2B and retail partners generate learnings while driving consumer adoption and excitement,” the company told its staff in the email late last week. The Urgent Company had always been intended as a temporary asset for Perfect Day, the spokesperson added, with a plan to sell off its brands once they were large enough.Ī former employee told NOSH the closure was also a result of timing issues, with company executives surprised by retail reset cycles and the length of time it takes to get a new product into stores. There’s not the luxury that there used to be to be able to expand and maximize opportunities in different adjacencies.” “There’s just a different level of focus that investors are expecting with their capital. “As you can imagine, the economic climate right now is different than it was even just two, three years ago,” the Perfect Day spokesperson told NOSH. Some of the impetus to do so may have also come from investor pressure. When asked why the company chose to shutter the consumer business rather than just sell it to begin with, the spokesperson said that the company had explored that option, but, rather than continue to support a large team, the company decided to stop devoting resources to the business. Perfect Day “will not be investing any more time or resources into brands,” and is “look for places where we can be opportunistic in offering partner products” to retailers, the Q and A noted. ![]() The company is aiming to sell all or part of The Urgent Company and to have fully exited all consumer facing businesses by September, the Q&A stated. In order to avoid retail service disruptions and sell through existing inventory, a small number of TUC employees will remain with Perfect Day as consultants. The Urgent Company team’s official last day is September 4 however, they are no longer involved in the day-to-day operations of the business. ![]() A Q&A document sent to remaining employees last week noted that reductions in force had also impacted some team members in B2B, BaaS, and corporate services but it remains unclear if that is in reference to last week’s layoffs or prior terminations. Other rounds of layoffs have occurred over the past year, but a Perfect Day spokesperson declined to share exact numbers. ![]() No cuts were made to the company’s India-based manufacturing business, which it acquired in 2022. In total, 134 Perfect Day employees were laid off: 122 in the U.S. A spokesperson for Perfect Day said that while the company maintains its headquarters in the Bay Area, the layoffs were conducted digitally due to remote workers. On the morning of July 6, employees within the consumer division were informed of their termination via a letter sent to their personal email accounts and were simultaneously locked out of their computers and work software. Team members began to suspect changes were afoot the week of June 26, when Alex Brittian, Perfect Day’s consumer division president for Asia and the U.S, was laid off, a source formerly with Perfect Day told NOSH. “As part of this, we have to take the incredibly difficult step of parting ways with our talented B2C team members and reducing some Perfect Day positions to support this focused business.” “We refocus all efforts on our founding principles of R&D innovation and the resulting B2B partnership opportunities from our investment into the technology we have been building over the past nine years,” wrote president Narayan Tripunithura Mahadeva (who goes by TM).
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It offers a party system like a group feature in a social network platform made by a specific person, and other people can join this party by entering a unique code. ![]() Festify is a free music app that allows multiple people to join a single party. ![]() ![]() Michael Dobson is Director of the Shakespeare Institute in Stratford-upon-Avon and Professor of Shakespeare Studies, University of Birmingham. Use the promo code SHAKESPEARE for 14 days free. For the full course, subscribe today on Himalaya Learning. You can hear the third episode of this course for free below. In Part 3, Professor Dobson offers close-readings of some of the play’s most important speeches, including Brutus’s deliberation over Caesar’s assassination and the rival speeches given by Brutus and Antony to “Friends, Romans, countrymen” at Caesar’s funeral - speeches that display the potential power of rhetoric. Professor Dobson also discusses the Roman values that the characters strive to embody and how these values generate friendships, rivalries, and violence. Is Caesar, in Shakespeare’s story, really a tyrant who needed to be killed? Is Brutus a noble political hero or a misguided egoist? With Professor Dobson, you’ll discover how Shakespeare restructured this familiar story to make easy judgments impossible. Part 2 focuses on the play’s key interpretive questions: how we are invited to judge the central characters. This summary is told using the language of the play itself, placing key quotations in context to help you understand where these lines come from and what they mean. Professor Dobson discusses the Roman history behind Julius Caesar and the cultural role of classical Rome during the Renaissance, when Shakespeare was writing. In Part 1, you’ll be guided through a detailed account of the story with commentary by Michael Dobson, Director of the Shakespeare Institute in Stratford-upon-Avon and Professor of Shakespeare Studies, University of Birmingham. You’ll also hear the play’s key speeches performed and analyzed by world-class Shakespearean actors and literary scholars. In this course, you’ll learn how Shakespeare dramatized the historical event of Caesar’s assassination in 44 BCE, and particularly how he linked refined political rhetoric, aspiration toward Roman ideals, and acts of savage violence. In this tense political thriller, the Roman senator Brutus must decide whether to assassinate the powerful military general Julius Caesar in order to save Roman Republic - and the audience must decide whether Brutus made the right choice. Julius Caesar is one of Shakespeare’s most famous plays, telling the story of one of history’s most famous events. Oxford: Oxford University Press, 1984, reissued 2008. London: Bloomsbury Publishing Plc, 1998, reprinted 2018. Howard, Katharine Eisaman Maus and Gordon McMullan. Edited by Stephen Greenblatt, Walter Cohen, Suzanne Gossett, Jean E. Maus, Katharine Eisaman, “Introduction,” Julius Caesar, in Shakespeare, William. “ Julius Caesar: A Modern Perspective.” Folger Shakespeare Library. The characters’ distinctive rhetorical styles and where they draw their persuasive powerĮpisode 1: Julius Caesar - the Story and the ContextĮpisode 2: Julius Caesar - the Characters and the Questions ![]() The strategies Shakespeare uses to render historical figures as morally and psychologically complex dramatic characters ![]() The story of Julius Caesar and its relationship to Roman history and Renaissance culture Through our network of niche job boards (, , and ) you can search for other ministry opportunities. 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Float the gel in transfer buffer while preparing the transfer sandwich.Notch the top left corner to indicate gel orientation.Start by removing the gel from its cassette and cutting the top portion containing the wells.The steps of the traditional wet transfer method are as follows: Transfer can occur under wet or semi-dry conditions. This is based upon the same principle as the previous step in which an electric field is charged to move the negative proteins towards a positive electrode. In this next step, we will transfer separated proteins out of the gel into a solid membrane or blot. During this time the negatively charged proteins in each sample will migrate toward the positively charged electrode making their way through the polyacrylamide gel matrix. Most units typically run 45-60 minutes at 200 volts or until the loading buffer reaches the bottom of the gel. Close the electrophoresis unit and connect it to a power supply.The ladder will allow you to monitor protein separation during electrophoresis and subsequently verify protein weight in your sample during later analysis. Load your samples into the wells and load a pre-stained molecular weight ladder into one well.Rinse the wells of the gel with a running buffer and add a buffer to the chambers. Prepare your gel by inserting it into the electrophoresis apparatus and filling it with a running buffer that is appropriate for your gel chemistry.Therefore higher percentage of gels are better for low molecular weight proteins, a low percentage of gel are useful for large proteins and gradient gels can be used for proteins of all sizes due to their varying range in pore size. The higher the acrylamide percentage the smaller the pore size of the gel matrix. Gels are available in fixed percentages or gradients of acrylamide. To do this, we load our previously prepared protein samples into a commercially available polyacrylamide gel. In this step, we will separate the individual proteins in our sample lysate based on their molecular weight using a positive electrode to attract a negatively charged protein. Now the sample is ready to load into an SDS page gel. Vortex each sample and incubate at 95 degrees Celsius for five minutes to completely denature the proteins. bromophenol blue to visualize the lysate and an ionic buffer.glycerol to allow the samples to sink into each well,.SDS to assist in denaturing and to provide a net negative charge to the protein,.beta-mercaptoethanol, or DTT, to reduce disulfide bridges between cysteines,.To reduce and denature samples dilute each in a loading buffer such as Laemmli sample buffer. These conditions will allow proteins to be separated by their molecular weight rather than their native conformational shape or charge. Western blots are typically performed under reduced and denatured conditions. The supernatant is the lysate which we will use for further processing. The cell mixture is centrifuged and the pellet is discarded. Cells are lysed by incubating on ice and later applying shear pressure using a pipette. Lysis buffer should contain protease inhibitors to prevent the degradation of the protein of interest. (The choice of lysis buffer largely depends on the localization of the protein of interest, solubilization of membrane-bound proteins requires stronger extraction detergents compared with isolated cytoplasmic proteins).Īlways use freshly prepared protease inhibitors, keep samples on ice and work quickly. Take the sample, add ice-cold PBS and lysis buffer such as RIPA buffer which is a commonly used buffer for maximum protein yield. Western blotting procedures include the following steps: Tissue Preparation (preparation of sample lysate): ![]() You may need to have two (or more) blood tests for accurate results. Some genetic disorders, including the inherited disease familial hypertriglyceridemia and familial combined hyperlipidemia (triglyceride and LDL are both elevated).Ī blood test can reveal whether or not you have high triglycerides.Hypothyroidism (insufficient production of thyroid hormones).Adverse side effect of particular medications.Occasionally, the trigger is an underlying condition such as: In many cases, habitual overeating causes high triglycerides. Low HDL levels are a known risk factor in the development of heart disease. Studies show that many people with high triglycerides have low levels of HDL – the ‘good’ cholesterol that helps remove fat from the artery. High triglycerides contribute to the development of atherosclerosis. A complete blockage can cause life-threatening conditions including heart attack or stroke. Blood flow is restricted through these narrowed blood vessels. Over time, raised LDL causes fatty plaques to form on blood vessel walls. The two types of cholesterol are high density lipoprotein (HDL) and low density lipoprotein (LDL). Triglycerides are a type of fuel, while cholesterol is needed for various metabolic processes such as making particular hormones and building cells. However, the body uses triglycerides and cholesterol differently. Like triglycerides, cholesterol is a fatty substance that circulates in the blood. Low HDL (high density lipoprotein) cholesterol.Higher than normal blood glucose levels.Central (abdominal) obesity – excess fat in and around the stomach (abdomen).A person with metabolic syndrome has an increased risk of developing diabetes, stroke or heart disease.Ī person is classed as having metabolic syndrome when they have any three of the following factors: High triglyceride levels are associated with a collection of disorders known as ‘ metabolic syndrome’. High triglycerides are also known as hypertriglyceridemia. High blood triglycerides are linked with an increased risk of health conditions including heart disease. If you regularly eat more kilojoules than you burn, you will be overweight or obese and may have raised triglyceride levels circulating in your blood. Any leftovers are stored in body fat to fuel the body between meals. Once digested, triglycerides circulate in the bloodstream to be used as energy by the cells. Both animal and vegetable fats contain triglycerides. Triglycerides are a common type of fat that accounts for about 95 per cent of all dietary fats. ![]() Sortino - This ratio is a modification of the Sharpe Ratio that uses the downside deviation instead of the total standard deviation of the mutual fund.Sharpe Ratio - This ratio shows how much extra returns are being generated for the additional risk being taken by the fund.The standard deviation shows much the fund returns have moved up and down from their average in the past Standard Deviation - It is the measure of fund volatility.There are 4-5 key risk measurement ratios that are used to compare mutual funds ![]() AMCs can charge whatever they want as long as they don't cross the upper limit defined by the regulator There is a ceiling set by SEBI on how much AMCs can charge. The Expense Ratio is the annual percentage the fund house charges you for managing your money. All Mutual Funds are benchmarked to a particular index and this data shows how much more or less the fund has delivered compared to that index. The second criteria which are most often used are how the fund has performed against its benchmark. In most cases, prospective investors look at the 1 year, 3 years, and 5-year returns of different schemes to compare them. This is by far the most common criteria used to compare mutual fund schemes. There is the list of the most commonly used ones There are many different parameters that are available and are used while comparing Mutual Funds. Having a clear idea about how to compare mutual funds performance and other features can help investors choose schemes that are in line with their investment goals and help them design an investment portfolio that minimizes overall risk and maximizes returns. This is where comparing types of mutual funds can help you select the right schemes to invest in. Also considering that investing involves a long-term commitment using your hard-earned money, choosing the right Mutual Fund is critical for achieving long-term goals. While having options to choose from is definitely a good thing, having too many options can make fund selection quite difficult. This is because each of the 3 categories has multiple sub-categories with multiple Fund Houses offering similar funds. While the three broad choices of Equity, Debt, and Hybrid Funds seem simple enough, there are currently over 2000 Mutual funds in India that investors can choose to invest in. Investors are spoilt for choice when it comes to choosing which Mutual Fund to invest in. Why Do You Need to Compare Mutual Fund Schemes? ![]() Packages going to certain places (including Alaska, Hawaii, and offshore destinations), or containing hazardous materials or live animals may receive slower service. ![]() In most cases, the expected delivery date that is printed on your receipt or provided at checkout will reflect a delivery time of 1, 2, or 3 days based on origin, destination, and Post Office ™ acceptance time. For additional information, visit the Postage Calculator. After completing these final steps, ensure that the seal is unbroken and your stamp hasnt been canceled. Then finish off the addressed envelope with the recipients full name, title, company, city, state, zip code, and date. Scheduled delivery date and time depends on origin, destination, and Post Office ™ acceptance time. Dont forget to sign the letter or card Add your signature right below the printed text. If none of the choices matches your envelope size, scroll to the bottom of the list, click Custom size, and then type the dimensions of your. In the Envelope size box, click the choice that matches the size of your envelope. Click Options, and then click the Envelope Options tab. The sample is always attached to either the letter or the two page catalog. On the Mailings tab, in the Create group, click Envelopes. It consistently produces a very high conversion rate. See the International Mail Manual for availability and limitations of coverage. A sample in the envelope works wonders in getting people to open the letter, and then to respond to it. Purchased insurance replaces ordinary indemnity coverage. Not available with Flat Rate envelopes and small Flat Rate boxes or when shipping to certain destinations. For just 1 0.63 First-Class Mail Forever stamp, you can send 1 oz (about 4 sheets of regular, 8-1/2' x 11' paper in a rectangular envelope) to anywhere in the U.S. For large envelopes (flats) up to 15.994 oz., postage prices vary based on weight and destination, starting from 2.90. Step 1: Choose Envelope or Postcard Envelopes are for sending flat, flexible things, like letters, cards, checks, forms, and other paper goods. For details regarding claim exclusions, see Sections 609.4.3 (Non-payable Claims), 609 (Filing Indemnity Claims for Loss or Damage), and 503.4 (Insured Mail) of the Domestic Mail Manual. letters or postcards around the world with one Global Forever ® stamp, which currently costs 1.45 and never expires, even if the postage price goes up. Available only with certain products and to certain domestic destinations. When addressing envelopes larger than regular letter-size envelopes, place the address to the right edge of the piece. TIP: Use the USPS Price Calculator tool to calculate your domestic postage costs. More than 2 cubic feet (3,456 cubic inches): add 25.00. For details on free Package Pickup, go to Schedule a Pickup. For expedited shipping (via Priority Mail Express or Priority Mail), you’ll pay more if your package measures: Between 22 and 30 inches long: add 4.00. |
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